锦鲤求职岗位描述
Senior Scientist (Gene Editing), LNP platform
Responsibilities：
1. CRISPR/Cas9 editing system development
• Design, develop, screen, and optimize CRISPR/Cas9 genome editing systems.
• Design and screen sgRNAs and optimize editing activity.
• Develop efficient and specific CRISPR/Cas9 editing strategies for different genomic targets.
• Select appropriate Cas9 systems and editing strategies according to specific applications.
• Assist in establishing assays for evaluating CRISPR/Cas9 editing efficiency.
• Identify and optimize critical factors affecting editing efficiency and specificity.
2. Knock-in Platform Development.
• Contribute to the development of CRISPR/Cas9-mediated precision knock-in (KI) technologies.
• Design and optimize different types of DNA donors.
• Investigate and develop different KI mechanisms and strategies, including but not limited to HDR-mediated KI and NHEJ/HITI-mediated KI.
• Explore other DNA donor-mediated KI strategies.
• Optimize donor architecture, donor length, homology arms, and target site design.
• Investigate the impact of sgRNA, Cas9, donor design, and delivery strategies on KI efficiency.
• Support the establishment of screening and quantitative analytical methods for different KI strategies.
• Optimize key KI parameters, including KI efficiency, precision, productivity, and specificity.
• Support the establishment of a versatile KI platform applicable to different genomic targets and tissue/cell types.
Qualifications:
1. PhD degree, preferably graduating in 2026 or recently graduated, in Biology, Molecular Biology, Biochemistry, Bioengineering, Pharmaceutical Sciences, Pharmacology, Gene Therapy, Genome Editing, or a related field.
2. Strong research experience in CRISPR/Cas-based genome editing during PhD training.Solid understanding of CRISPR/Cas9 mechanisms and genome editing technologies.
3. Research experience in at least one of the following areas:
• CRISPR/Cas9
• Genome editing
• Knock-in / Knock-out
• HDR
• NHEJ/HITI
• DNA donor design
• sgRNA design and screening
4. Hands-on experience in knock-in research is strongly preferred, particularly experience in DNA donor design and KI optimization.
5. Familiarity with genome editing analytical methods, including PCR, qPCR/ddPCR, and Sanger sequencing.Strong experimental design, data analysis, and scientific problem-solving skills.
6. Ability to conduct independent research and rapidly learn emerging genome editing and delivery technologies.
7. Strong English scientific literature reading and technical writing skills.